Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity
Mark Barton Frank , Qing Yang , Jeanette Osban , Joseph T Azzarello , Marcia R Saban , Ricardo Saban , Richard A Ashley , Jan C Welter , Kar-Ming Fung and Hsueh-Kung Lin
BMC Complementary and Alternative Medicine 2009, 9:6doi:10.1186/1472-6882-9-6
Published: 18 March 2009
Abstract (provisional)
Background
Originating from Africa, India, and the Middle East, frankincense oil has been important both socially and economically as an ingredient in incense and perfumes for thousands of years. Frankincense oil is prepared from aromatic hardened gum resins obtained by tapping Boswellia trees. One of the main components of frankincense oil is boswellic acid, a component known to have anti-neoplastic properties. The goal of this study was to evaluate frankincense oil for its anti-tumor activity and signaling pathways in bladder cancer cells.
Method
Frankincense oil-induced cell viability was investigated in human bladder cancer J82 cells and immortalized normal bladder urothelial UROtsa cells. Temporal regulation of frankincense oil-activated gene expression in bladder cancer cells was identified by microarray and bioinformatics analysis.
Results
Within a range of concentration, frankincense oil suppressed cell viability in bladder transitional carcinoma J82 cells but not in UROtsa cells. Comprehensive gene expression analysis confirmed that frankincense oil activates genes that are responsible for cell cycle arrest, cell growth suppression, and apoptosis in J82 cells. However, frankincense oil-induced cell death in J82 cells did not result in DNA fragmentation, a hallmark of apoptosis.
Conclusion
Frankincense oil appears to distinguish cancerous from normal bladder cells and suppress cancer cell viability. Microarray and bioinformatics analysis proposed multiple pathways that can be activated by frankincense oil to induce bladder cancer cell death. Frankincense oil might represent an alternative intravesical agent for bladder cancer treatment.
The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.
Thursday, March 19, 2009
Vitamin E and Prostate Cancer
Vitamin E and Prostate Cancer: A Proteomics Approach Using 2-D Gel Analysis Software
Proteomics can be a useful tool in understanding the anti-cancer activity of vitamin E.
By Christian M. Muenyi, Dr. William L. Stone, Dr. Hamid Kasmai, and Hongsong Yang
Various forms of vitamin E have been under intensive study as chemopreventive and chemotherapeutic agents for a number of cancers.1 Many in vitro, animal, and epidemiological studies have presented evidence of an anti-cancer activity for vitamin E, but there are few studies of vitamin E in prostate cancer,2 and the mechanisms by which forms of vitamin E induce apoptosis in cancer cells remains largely unknown.3 Therefore, proteomics may help to understand the molecular events associated with the cytotoxic effects of vitamin E on cancer cells.
Figure 1: Analysis of 2-D gel by Dymension software showing proteins that are up or down-regulated three hours after delta-tocotrienol treatment.
The purpose of the study was to characterize the proteomic changes occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol, a form of vitamin E. In this study, 2-D gel electrophoresis was used to detect changes in protein expression levels associated with this treatment. However, to determine which proteins in a complex 2-D gel image are being expressed requires specialist software to resolve protein spots accurately. Previously, using some 2-D analysis software packages, it was difficult and time consuming to manipulate gel images to obtain meaningful data. To overcome the analysis bottleneck, this article describes how Dymension (Syngene, Frederick, Md.), a 2-D gel image analysis software, can be used to rapidly show which proteins are up or down-regulated by treatment with delta-tocotrienol.
Proteomics can be a useful tool in understanding the anti-cancer activity of vitamin E.
By Christian M. Muenyi, Dr. William L. Stone, Dr. Hamid Kasmai, and Hongsong Yang
Various forms of vitamin E have been under intensive study as chemopreventive and chemotherapeutic agents for a number of cancers.1 Many in vitro, animal, and epidemiological studies have presented evidence of an anti-cancer activity for vitamin E, but there are few studies of vitamin E in prostate cancer,2 and the mechanisms by which forms of vitamin E induce apoptosis in cancer cells remains largely unknown.3 Therefore, proteomics may help to understand the molecular events associated with the cytotoxic effects of vitamin E on cancer cells.
Figure 1: Analysis of 2-D gel by Dymension software showing proteins that are up or down-regulated three hours after delta-tocotrienol treatment.
The purpose of the study was to characterize the proteomic changes occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol, a form of vitamin E. In this study, 2-D gel electrophoresis was used to detect changes in protein expression levels associated with this treatment. However, to determine which proteins in a complex 2-D gel image are being expressed requires specialist software to resolve protein spots accurately. Previously, using some 2-D analysis software packages, it was difficult and time consuming to manipulate gel images to obtain meaningful data. To overcome the analysis bottleneck, this article describes how Dymension (Syngene, Frederick, Md.), a 2-D gel image analysis software, can be used to rapidly show which proteins are up or down-regulated by treatment with delta-tocotrienol.
Friday, November 14, 2008
Study Fails to Find Fertility Drug-Cancer Link
June 15, 2004 -- Taking fertility drugs does not appear to increase a woman's risk of developing ovarian cancer, a new study from the National Cancer Institute suggests.
Researchers found no associations between the use of medications that stimulate ovulation and ovarian cancer in a study involving more than 12,000 women who had trouble conceiving. However, a slight increase in risk was seen among women followed for the longest time, but researchers say this increase may not be related to the use of fertility drugs.
"In general our findings are very reassuring," lead researcher Louise Brinton, PhD, tells WebMD. "We failed to confirm a major increase with the use of these drugs, but most of these women are still relatively young. For this reason, the association warrants further follow-up."
Early Findings Mixed
Since their introduction almost four decades ago, ovulation-stimulating drugs like Clomid have been prescribed to millions of infertile women. Previous studies assessing the impact of these drugs on ovarian cancer risk have been mixed, with one study suggesting up to a 27-fold increase in cancer risk among treated women who never got pregnant and other studies failing to find any link at all.
The NCI trial included 12,193 women who were seen at five infertility clinics throughout the country between 1965 and 1988. Of these, more than 4,000 women had used a fertility drug.
By 1999, 45 of the women had been diagnosed with ovarian cancer, which was almost twice the number expected in the general population.
But when the researchers adjusted for other ovarian cancer risk factors, the use of fertility drugs was not found to significantly increase cancer risk.
In other words, ovarian cancer risks were similar regardless of whether these women used fertility drugs or not.
Clomid users actually had slightly fewer ovarian cancers than would be expected in the general population, while users of the protein hormone gonadotropin had slightly more cancers.
Fifteen years after treatment, the odds of developing ovarian cancer more than doubled among the small group of gonadotropin users followed for many years.
"These women are only now beginning to reach the age range where ovarian cancer is most often diagnosed," Brinton says. "That is why it is important to continue following them."
Women Who Don't Get Pregnant
Fertility expert Alan DeCherney, MD, says recent studies are consistent in finding no link between fertility drug use and ovarian cancer risk. It is now clear that women who never achieve a pregnancy are at increased risk, however, and DeCherney says earlier studies failed to take this into consideration.
"We now know that women who fail to get pregnant -- with or without fertility treatments -- are at greater risk," says DeCherney, who is a professor of obstetrics and gynecology at UCLA School of Medicine and a past president of the American Society for Reproductive Medicine. "Since those who can't conceive are the ones who take fertility drugs, it is easy to see why the early studies made this association."
Wednesday, October 08, 2008
Evidence-Based Interventions for Chemotherapy-Induced Peripheral Neuropathy
Chemotherapy-induced peripheral neuropathy (CIPN) continues to be a significant, debilitating symptom resulting from the administration of neurotoxic chemotherapy for the treatment of cancer. CIPN is an important consequence of cancer treatment because of its potential impact on physical functioning and quality of life. Oncology nurses play an important role in assessing, monitoring, and educating clients about CIPN. Despite investigations concerning pharmacologic and nonpharmologic approaches to either preventing or minimizing the neurotoxicity resulting from certain chemotherapeutic agents, evidence to support the interventions is lacking. This article presents information concerning CIPN and summarizes the pharmacologic and nonpharmacologic approaches to the prevention and treatment of CIPN.
Alex writes: Acupuncture and some nutritional supplements I have found are very effective for this problem.
Wednesday, September 24, 2008
Tests Done During and After Breast Cancer Treatment
Here's a rundown of the tests you can anticipate both during and after treatment:
Click on the title of the post to go to the article.
Alex writes: In addition to standard medical tests. It may also be prudent to check vitamin and mineral status and other metabollic function tests to more objectively apply nutrition. One important test that is not commonly given is the 25 OH Vitamin D test. Vitamin D has a lot of research about its use in the treatment and prevention of cancer. A vitamin D test can generally be included in your standard blood work from your MD . Tests such as the Metametrix Comprehensive Metabolic Profile are urine and finger prick blood tests that do not require a phlebotomist that are available for purchase from my office.
A single urine specimen provides important information about:
• Insufficiencies of carnitine and NAC
• Specific markers of bacterial dysbiosis
• Lipoic acid and CoQ10 sufficiency
• Functional status of B-vitamins
• Neurotransmitter metabolism
• Mitochondrial energy production
• Methylation sufficiency status
• Detoxification adequacy
• Oxidative stress and antioxidant sufficiency
Urine Lipid Peroxides
In its efforts to produce the chemical energy to power cells and fight infection, the body produces harmful free radicals. Antioxidant supplements can help protect cells from free radical damage that can result in oxidation of cell membranes, forming lipid peroxides. High levels of lipid peroxides are associated with cancer, heart disease, stroke, and aging.
BloodspotSM Fatty Acid Profile (not available in NY)
From a simple finger stick, this unique test measures the ratio of the principle omega-6 (AA) and omega-3 (EPA) fatty acids. The AA/EPA ratio is a measure of the body’s eicosanoid balance, detecting “silent” inflammation that can lead to heart disease and other chronic and inflammatory processes. Also included is an Index of Omega-3 Fatty Acids: the sum of EPA and DHA as a percent of total fatty acids. Studies have shown that this index can be used to estimate a person’s risk of dying from coronary heart disease (CHD). A bloodspot Index of Omega-3 Fatty Acids greater than 1.6 is associated with the greatest cardioprotection, whereas an
index less than 0.75 is associated with the least cardioprotection. With many consumers loading up indiscriminately on fish oils, this profile can also detect omega-3 dominant patterns which can lead to increased peroxidation and immune suppression.
BloodspotSM IgG Food Antibody Profile (not available in NY)
Researchers estimate that at least 60% of the U.S. population suffers from unsuspected food reactions that can cause or complicate health problems. These reactions are difficult to identify since they can occur hours or even days after consumption of an offend-
ing food. In some cases, a person may eat a food for several days before developing a reaction to it, so they may not realize the link between the food and their symptoms. Symptoms can be extraordinarily diverse, ranging from arthritis to eczema to migraines.
For that reason, many health professionals routinely consider food allergies or intolerances when evaluating a patient’s health problems. The BloodspotSM IgG Food Antibody Profile tests for sensitivity to 30 of the most commonly positive food antigens, all
from a single finger stick.
Click on the title of the post to go to the article.
Alex writes: In addition to standard medical tests. It may also be prudent to check vitamin and mineral status and other metabollic function tests to more objectively apply nutrition. One important test that is not commonly given is the 25 OH Vitamin D test. Vitamin D has a lot of research about its use in the treatment and prevention of cancer. A vitamin D test can generally be included in your standard blood work from your MD . Tests such as the Metametrix Comprehensive Metabolic Profile are urine and finger prick blood tests that do not require a phlebotomist that are available for purchase from my office.
A single urine specimen provides important information about:
• Insufficiencies of carnitine and NAC
• Specific markers of bacterial dysbiosis
• Lipoic acid and CoQ10 sufficiency
• Functional status of B-vitamins
• Neurotransmitter metabolism
• Mitochondrial energy production
• Methylation sufficiency status
• Detoxification adequacy
• Oxidative stress and antioxidant sufficiency
Urine Lipid Peroxides
In its efforts to produce the chemical energy to power cells and fight infection, the body produces harmful free radicals. Antioxidant supplements can help protect cells from free radical damage that can result in oxidation of cell membranes, forming lipid peroxides. High levels of lipid peroxides are associated with cancer, heart disease, stroke, and aging.
BloodspotSM Fatty Acid Profile (not available in NY)
From a simple finger stick, this unique test measures the ratio of the principle omega-6 (AA) and omega-3 (EPA) fatty acids. The AA/EPA ratio is a measure of the body’s eicosanoid balance, detecting “silent” inflammation that can lead to heart disease and other chronic and inflammatory processes. Also included is an Index of Omega-3 Fatty Acids: the sum of EPA and DHA as a percent of total fatty acids. Studies have shown that this index can be used to estimate a person’s risk of dying from coronary heart disease (CHD). A bloodspot Index of Omega-3 Fatty Acids greater than 1.6 is associated with the greatest cardioprotection, whereas an
index less than 0.75 is associated with the least cardioprotection. With many consumers loading up indiscriminately on fish oils, this profile can also detect omega-3 dominant patterns which can lead to increased peroxidation and immune suppression.
BloodspotSM IgG Food Antibody Profile (not available in NY)
Researchers estimate that at least 60% of the U.S. population suffers from unsuspected food reactions that can cause or complicate health problems. These reactions are difficult to identify since they can occur hours or even days after consumption of an offend-
ing food. In some cases, a person may eat a food for several days before developing a reaction to it, so they may not realize the link between the food and their symptoms. Symptoms can be extraordinarily diverse, ranging from arthritis to eczema to migraines.
For that reason, many health professionals routinely consider food allergies or intolerances when evaluating a patient’s health problems. The BloodspotSM IgG Food Antibody Profile tests for sensitivity to 30 of the most commonly positive food antigens, all
from a single finger stick.
Tuesday, September 23, 2008
Acupuncture Reduces Painful Side Effects of Breast Cancer Treatments
See Video and short article from ABC World News Tonight an interview with the studies lead author, Dr. Eleanor Walker, Radiation Oncologist
click on title of article to see the video.
For an article about the study click here.
Thursday, August 07, 2008
American Cancer Society study finds high use of complementary methods among cancer survivors
Public release date: 4-Aug-2008
[ Print Article | E-mail Article | Close Window ]
Contact: David Sampson
david.sampson@cancer.org
American Cancer Society
[ Print Article | E-mail Article | Close Window ]
Contact: David Sampson
david.sampson@cancer.org
American Cancer Society
American Cancer Society study finds high use of complementary methods among cancer survivors
A new study from researchers at the American Cancer Society finds many cancer patients use complementary and alternative methods, most often prayer, relaxation, supplements, meditation, and massage. Meanwhile, the use of other methods, such as biofeedback, homeopathy, and acupressure, are relatively uncommon. The study, appearing in the American Cancer Society peer review journal CANCER, also finds women, younger survivors, whites, individuals with higher income, and those with more education were more likely to use complementary methods (CM).
The study confirmed the findings of previous surveys that found cancer patients use the same complementary methods used among the general population and among people with other chronic diseases, such as spiritual practices, relaxation methods, and dietary supplements. "Our study found that several CM types are used by nearly half of cancer survivors," said Ted Gansler, M.D., American Cancer Society and study co-author. "Surprisingly, other methods such as acupuncture and hypnosis were used by fewer than 2% of cancer survivors, even though recent studies found them to be useful in relieving some cancer-related symptoms, such as pain. We also found that the use of various CM types is significantly influenced by gender, race, age, education, cancer type, and how far the cancer had spread."
Previous studies on the use of complementary methods have relied on data from patients at a single or a few centers, which seldom provide nationally representative samples. Those studies have used small sample sizes, have focused on a single or few cancer types, and have involved patients in active treatment. For the current study, researchers used data from more than 4,000 survivors of ten different cancers participating in the American Cancer Society's Study of Cancer Survivors-I (SCS-I) who were surveyed 10 to 24 months after diagnosis. Survivors were asked if they had used any of 19 CMs to deal with their cancer. The CMs most frequently reported were prayer/spiritual practice (61.4%), relaxation (44.3%), faith/spiritual healing (42.4%), nutritional supplements/vitamins (40.1%), meditation (15%), religious counseling (11.3%), massage (11.2%), and support groups (9.7%). The least prevalent CMs were hypnosis (0.4%), biofeedback therapy (1.0%), and acupuncture/acupressure (1.2%).
The study also found cancer type was a significant predictor of CM use. Melanoma and kidney cancer survivors were least likely to use CMs, whereas breast and ovarian cancer survivors were most likely to use them.
Alex writes: My eye immediately went to the poor showing of acupuncture and the discussion that follows. "Surprisingly, other methods such as acupuncture and hypnosis were used by fewer than 2% of cancer survivors, even though recent studies found them to be useful in relieving some cancer-related symptoms, such as pain." So the evidence is good for its use but it is not used much. I don't exactly know what to make of this finding. Whatever the case there is a tremendous benefit to improving quality of life, recovery from chemotherapy and radiation and even improving the effectiveness of chemotherapy and radiation with acupuncture and herbs. Anyone else have a read on this?
Sunday, August 03, 2008
Vaccines, Immune Function and Disease
Endotoxins and vaccines may, unexpectedly, trigger the immune system to prevent cancer. The evidence in this article is interesting, and compelling. Click on the title of the article to follow the link.
Many believers in alternative medicine, especially many of our patients, devoutly avoid vaccinations. The point of this article is not to argue the pros and cons of vaccinations but to review some fascinating data on immune function and disease.
Before scientists knew that endotoxins in cow manure dust stimulated the immune system and lowered risk of lung cancer, they already knew of another dust that was protective. Back in the 1970s, data showed that people working in cotton factories had a lower risk of lung cancer. Cotton dust, like cow manure, contains endotoxins.
Harvey Checkoway at the University of Washington has published a series of papers about this. He and his colleagues have followed the cancer rates of female cotton textile workers in Shanghai, China, for years. The more dust these women breathed, the lower their incidence of cancers. Note that I wrote the plural – cancers. The women had a lower risk for several cancer types including pancreatic, liver, lung and breast cancer.
Not all dust is protective. For example, workers in paper mills are at greater than average risk for cancer. This increase might be due to the dust or because of exposure to some chemicals used in the mill. Similar increased risk occurs in hard-metal workers.
Friday, July 18, 2008
Antacids for indigestion
If you’re about to reach for that bottle of antacids after the Christmas blow-out - think again. New research suggests that the world’s most popular drugs, used for heartburn and indigestion, can double the risk of pneumonia.
This is because these drugs suppress gastric acids, thereby allowing viruses and bacteria in the upper gastrointestinal tract to migrate up into the respiratory tract. read the whole study.
Alex says: Antacids do a whole lot more than increase the potential for infection. They block the breakdown of proteins and inhibit mineral absorption (not good if you have osteoporosis). There are other ways to handle reflux that get at the root of the inflammatory problem without just symptomatically covering up the problem
Wednesday, July 02, 2008
Vitamin D May Help Patients Survive Cancer
Vitamin D May Help Patients Survive Cancer
New Report Suggests Sunshine Vitamin May Have Significant Cancer Benefits
When Joanna Fuchs was diagnosed with colon cancer last year, a blood test revealed she was severely deficient in vitamin D.
Natural sunlight is one way you can get vitamin D.
(Jean Desy/First Light/Getty Images)"I was obviously very concerned and very worried," Fuchs said.
So, too, was her husband, Dr. Charles Fuchs of the Dana Farber Cancer Institute, who is the senior author of a study published today that found colon cancer patients deficient in vitamin D were almost twice as likely to die over a 10-year period than patients with healthy levels of the nutrient.
"These findings make considerable sense," Dr. Fuchs said, "because in the laboratory we find that vitamin D reduces the growth of colon cancer cells, prevents its spread to other organs, and actually reduces the growth of blood vessels to these tumors."
Related
Fuchs' work is just the latest in a wave of new studies on vitamin D. Vitamin D deficiency has been linked to a greater risk of autoimmune diseases, including type 1 diabetes, multiple sclerosis and rheumatoid arthritis. It has also been linked to a doubling of heart attack risk among men, a 73 percent greater risk of dying from breast cancer, and more frequent asthma attacks among children.
"Vitamin D seems to be very helpful in making your immune system do what it is supposed to do," Dr. Tanya Edwards, director of Integrative Medicine at the Cleveland Clinic.
The government recommends getting 200 to 600 international units of vitamin D a day. But those levels were set 11 years ago, primarily to keep bones healthy. To prevent other diseases, many researchers now say more is needed.
Alex's comment: I recommend 2000 IU's and if you have an autoimmune disease 5,000 Iu's. It is a cheap add on to a blood test and well worth getting checked out.
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