Gao D, Lin JM, Zheng LP. [Experimental study on effect of xue fu zhu yu
decoction on bone marrow hematopoietic stem cells of mice] [Article in
Chinese] Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Jun;27(6):527-
Fujian College of Traditional Chinese Medicine, Fuzhou. OBJECTIVE: To
investigate the effect of Xuefu Zhuyu Decoction (XFZYD) on the number,
phenotype, cell cycle and colony formation of bone marrow hematopoietic
stem cells (HSC) in mice. METHODS: Kunming mice were randomly divided
into 4 groups: the control group, the low- (3.25 g/kg), middle- (6.5 g/kg) and
high-dose (13.0 g/kg) XFZYD groups. After they were medicated by
gastrogavage respectively with saline or corresponding dose of XFZYD for 7
days, their bone marrow HSC were separated and counted. The phenotype
Sca and cell cycle of HSC were detected by flow cytometer, and the colony
formation was determined with semisolid methyl media culture. RESULTS:
No obvious difference in the number of mononuclear cell, suspended cell
and colony production was found among all the groups (P > 0.05); while the
expression of CD34 and Sca-1 increased in the low-dose XFZYD group, but
in the middle-dose XFZYD group increase only showed in Sca-1 expression.
CONCLUSION: XFZYD plays a role of removing blood stasis and promoting
regeneration through improving hematopoietic function by means of
increasing the number and enhancing the function of premature HSC. PMID:
17633366 [PubMed - in process]
