- Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Jun;27(6):527-30.
[Experimental study on effect of xuefu zhuyu decoction on bone marrow hematopoietic stem cells of mice][Article in Chinese]
Fujian College of Traditional Chinese Medicine, Fuzhou.
OBJECTIVE: To investigate the effect of Xuefu Zhuyu Decoction (XFZYD) on the number, phenotype, cell cycle and colony formation of bone marrow hematopoietic stem cells (HSC) in mice. METHODS: Kunming mice were randomly divided into 4 groups: the control group, the low- (3.25 g/kg), middle- (6.5 g/kg) and high-dose (13.0 g/kg) XFZYD groups. After they were medicated by gastrogavage respectively with saline or corresponding dose of XFZYD for 7 days, their bone marrow HSC were separated and counted. The phenotype Sca and cell cycle of HSC were detected by flow cytometer, and the colony formation was determined with semisolid methyl media culture. RESULTS: No obvious difference in the number of mononuclear cell, suspended cell and colony production was found among all the groups (P > 0.05); while the expression of CD34 and Sca-1 increased in the low-dose XFZYD group, but in the middle-dose XFZYD group increase only showed in Sca-1 expression. CONCLUSION: XFZYD plays a role of removing blood stasis and promoting regeneration through improving hematopoietic function by means of increasing the number and enhancing the function of premature HSC.
PMID: 17633366 [PubMed - in process]
Monday, July 23, 2007
This study demonstrates that the classic chinese herbal formula Xue Fu Zhu Yu Tang (Remove Stasis from the blood mansion) increases hematopoietic stem cells. In TCM terms this formula activates blood and has many uses for patterns of blood stagnation. Modifications of this formula could really be of use for folks that are suffering from bone marrow suppressed anemias. Good to know. Alex